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Table 1 Overview of the investigated genes of interest as well as the used reference genes, their gene symbol, name and primers

From: Culture-induced changes in mRNA expression levels of efflux and SLC-transporters in brain endothelial cells

Gene symbolGene namePCR efficiencyProduct size (Bp)Primer sequence (5′ to 3′)
HPRT1Hypoxanthine guanine phosphoribosyl transferase 12.03144F: CGT GGT GAT TAG CGA TGA TG
R: TTC ATC ACA TCT CGA GCC AG
ACTBβ-actin1.91426F: AGG CTG TGC TGT CCC TGT AT
R: AGG TAG TTT CGT GAA TGC CG
GAPDHGlyceraldeyde-3-phosphate dehydrogenase1.93102F: CGA CCA CTT TGT CAA GCT CA
R: GGA CCT TAC TCC TTG GAG GC
VWFvon Willebrand Factor1.92126F: TCT CAC GAG ACT GCA ACA CC
R: TGA AGT GCC TGT CGT CAA AG
ALPAlkaline phosphatase1.98139F: ATC GGT ACC TGT TTT GCC AG
R: TGA TGA CGT TCT TAG CCA CG
TPATissue plasminogen activator1.96103F: TCT TCT CCG TTC TTT TCC GA
R: TGT TGG TGA CGG TCC TGT TA
CLDN1Claudin-12.06122F: CCG TTG GCA TGA AGT GTA TG
R: CCA TGC TGT GGC AAC TAA AA
CLDN5Claudin-52.06122F: CAG AAG TAC GAG CTG GGA GC
R: TAC TTC ACC GGG AAG CTG AA
OCLNOccludin2.05143F: CCG GAA GAT GAA ATT CTC CA
R: GTT CCA TAG CCT CTG TCC CA
TJP1Zonula occludens 1
(Zo-1)
2.08148F: CGA CCA GAT CCT CAG GGT AA
R: GGA TTC TAC GAT GCG ACG AT
F11RJunctional adhesion molecule 1
(JAM-1)
1.94113F: TGC TGA CCT GCT CAG AGA GA
R: GGA AGA GTT GCT GAA GGC AC
ABCB1P-glycoprotein
(Pgp)
1.99127F: CGG GAC AGA AAG CTC AGT TC
R: TAA TGG CGC AAA ATA CAC CA
ABCG2Breast cancer resistance protein
(BCRP)
2.08139F: CCA GGC GTT CAT TCA AAA AT
R: GCT CTG TTC TGG ATT CCT GC
ABCC1Multidrug resistance protein 1
(MRP-1)
2.03106F: GTT CCC CTC AAT GCT GTG AT
R: TCC CGT TGA GAA TTT CGT TC
ABCC4Multidrug resistance protein 4
(MRP-4)
1.95124F: ATT AAA CGC CTG GAA TGT GC
R: GTG CAT TGA ACA GCT CCT GA
SLC2A1Glucose transporter 1
(GLUT-1)
1.92139F: TAC CCC AAG AGG TGG CTA TG
R: CTG GTC TCA GGC AAG GAA AG
SLC7A5L-type amino acid transporter 1
(LAT-1)
1.91111F: TAG CCA ATC TGG ATC CCA AG
R: TCA AGT AAT TCC ATC CCC CA
SLC16A1Monocarboxylate transporter 1
(MCT-1)
1.91143F: TTT ACG CCT TTG ATT CCC AG
R: TCC CTA TTG GTT TCT GTG GG
  1. The primer sequence, PCR efficiency and product size are listed for each of the designed primer sets
  2. PCR Polymerase chain reaction, Bp base pairs, F forward, R reverse