Table 1 Overview of the investigated genes of interest as well as the used reference genes, their gene symbol, name and primers
Gene symbol | Gene name | PCR efficiency | Product size (Bp) | Primer sequence (5′ to 3′) |
|---|---|---|---|---|
HPRT1 | Hypoxanthine guanine phosphoribosyl transferase 1 | 2.03 | 144 | F: CGT GGT GAT TAG CGA TGA TG R: TTC ATC ACA TCT CGA GCC AG |
ACTB | β-actin | 1.91 | 426 | F: AGG CTG TGC TGT CCC TGT AT R: AGG TAG TTT CGT GAA TGC CG |
GAPDH | Glyceraldeyde-3-phosphate dehydrogenase | 1.93 | 102 | F: CGA CCA CTT TGT CAA GCT CA R: GGA CCT TAC TCC TTG GAG GC |
VWF | von Willebrand Factor | 1.92 | 126 | F: TCT CAC GAG ACT GCA ACA CC R: TGA AGT GCC TGT CGT CAA AG |
ALP | Alkaline phosphatase | 1.98 | 139 | F: ATC GGT ACC TGT TTT GCC AG R: TGA TGA CGT TCT TAG CCA CG |
TPA | Tissue plasminogen activator | 1.96 | 103 | F: TCT TCT CCG TTC TTT TCC GA R: TGT TGG TGA CGG TCC TGT TA |
CLDN1 | Claudin-1 | 2.06 | 122 | F: CCG TTG GCA TGA AGT GTA TG R: CCA TGC TGT GGC AAC TAA AA |
CLDN5 | Claudin-5 | 2.06 | 122 | F: CAG AAG TAC GAG CTG GGA GC R: TAC TTC ACC GGG AAG CTG AA |
OCLN | Occludin | 2.05 | 143 | F: CCG GAA GAT GAA ATT CTC CA R: GTT CCA TAG CCT CTG TCC CA |
TJP1 | Zonula occludens 1 (Zo-1) | 2.08 | 148 | F: CGA CCA GAT CCT CAG GGT AA R: GGA TTC TAC GAT GCG ACG AT |
F11R | Junctional adhesion molecule 1 (JAM-1) | 1.94 | 113 | F: TGC TGA CCT GCT CAG AGA GA R: GGA AGA GTT GCT GAA GGC AC |
ABCB1 | P-glycoprotein (Pgp) | 1.99 | 127 | F: CGG GAC AGA AAG CTC AGT TC R: TAA TGG CGC AAA ATA CAC CA |
ABCG2 | Breast cancer resistance protein (BCRP) | 2.08 | 139 | F: CCA GGC GTT CAT TCA AAA AT R: GCT CTG TTC TGG ATT CCT GC |
ABCC1 | Multidrug resistance protein 1 (MRP-1) | 2.03 | 106 | F: GTT CCC CTC AAT GCT GTG AT R: TCC CGT TGA GAA TTT CGT TC |
ABCC4 | Multidrug resistance protein 4 (MRP-4) | 1.95 | 124 | F: ATT AAA CGC CTG GAA TGT GC R: GTG CAT TGA ACA GCT CCT GA |
SLC2A1 | Glucose transporter 1 (GLUT-1) | 1.92 | 139 | F: TAC CCC AAG AGG TGG CTA TG R: CTG GTC TCA GGC AAG GAA AG |
SLC7A5 | L-type amino acid transporter 1 (LAT-1) | 1.91 | 111 | F: TAG CCA ATC TGG ATC CCA AG R: TCA AGT AAT TCC ATC CCC CA |
SLC16A1 | Monocarboxylate transporter 1 (MCT-1) | 1.91 | 143 | F: TTT ACG CCT TTG ATT CCC AG R: TCC CTA TTG GTT TCT GTG GG |