Fig. 3

Angiomodulin antagonizes VEGF-A-induced peripheral vascular hyperpermeability. AGM’s effect on VEGF-A-induced vascular permeability was tested with the Miles assay. Recombinant VEGF-A and AGM proteins were injected sub-dermally to mice that received Evans-Blue tracer injection intravenously. Skin biopsy was taken from the injection site. The tracer was extracted and tracer levels were quantified with spectrophotometric absorbance. Relatively low VEGF-A concentrations (250 pg/ml) induced approximately 20% increase in vascular hyperpermeability over control (saline). Relatively low AGM concentrations (250 pg/ml), did not induced significant permeability. Injections of VEGF-A together with AGM, abolished VEGF-A-induced hyperpermeability (differences in leakage level between VEGF-A and the combined VEGF-A/AGM was statistically significant P < 0.05). Data represents permeability fold induction over saline control (indicated as the Permeability Factor). N = 4 mice. All data are mean ± sem. Statistical significance determined by a non-parametric Mann–Whitney U test