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Table 3 Antibodies for Western blotting analysis

From: Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity

Targets

Manufacturer

Catalog number

Species

Dilution

Occludin

Fisher Scientific

71-1500

Rabbit

1:500

ZO-1

Fisher Scientific

33-9100

Mouse

1:500

Claudin-5

Fisher Scientific

35-2500

Mouse

1:500

GAPDH (HRP)

Wako

35-2500

Mouse

1:1000

Anti-Mouse (HRP)

Abcam

ab6789

Goat

1:5000

Anti-Rabbit (HRP)

Abcam

ab6721

Goat

1:5000