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Table 2 Antibodies for immunofluorescence analysis

From: Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity

TargetsManufacturerCatalog numberSpeciesDilution
CDH5Santa Cruzsc-9989Mouse1:25
P-gpAbcamab10333Mouse1:25
BCRPAbcamab3380Mouse1:50
OccludinFisher Scientific71-1500Rabbit1:50
ZO-1Fisher Scientific33-9100Mouse1:100
Claudin-5Fisher Scientific35-2500Mouse1:50
Anti-Rabbit (Alexa Flour 488)Fisher ScientificA-21206Donkey1:200
Anti-Mouse (Alexa Fluor 568)Fisher ScientificA-11004Goat1:200