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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity

Fig. 4

Analyses of P-gp and BCRP functions in LN221F- and Matrigel-iBMELCs. a Relative intracellular accumulation of rhodamine 123. The iBMELCs were incubated with 10 μM rhodamine 123 in the absence or presence of 10 μM CsA for 60 min at 37 °C. Relative fluorescence intensity values were normalized to the condition without inhibitor (set to 1). Data are presented as the mean ± SD (n = 6; **p < 0.01; Student’s t-test). b Relative intracellular accumulation of Hoechst 33342. The iBMELCs were incubated with 10 μM Hoechst 33342 in the absence or presence of 20 μM Ko 143 for 60 min at 37 °C. Relative fluorescence intensity values were normalized to the condition without inhibitor (set to 1). Data are presented as the mean ± SD (n = 6; **p < 0.01; Student’s t-test)

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Fluids and Barriers of the CNS

ISSN: 2045-8118