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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Laminin 221 fragment is suitable for the differentiation of human induced pluripotent stem cells into brain microvascular endothelial-like cells with robust barrier integrity

Fig. 3

Characterization of LN221F- and Matrigel-iBMELCs. a Relative mRNA expression levels of CDH5, MDR1, BCRP, ZO-1, occludin, GLUT1, and LAT1 in LN221F- and Matrigel-iBMELCs derived from 610B1 on day 10. The values are normalized to those of HPRT1. The relative mRNA expression levels of Matrigel-iBMELCs were defined as 1. Data are presented as the mean ± SD (n = 3; N.S. = not significant; Student’s t-test). hBMECs: n = 1. b Immunofluorescence analysis of CDH5 (orange), P-gp (orange), BCRP (orange), ZO-1 (orange), occludin (green), and claudin 5 (orange) expression in LN221F- and Matrigel-iBMELCs derived from 610B1 on day 10. DAPI = blue. Scale bars = 50 μm

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