Fig. 4

Role of ischemic autophagy in OGD-induced dysfunction in brain endothelial cells. bEnd.3 cells were treated with 3-MA for 1 h prior to and during the 18 h OGD exposure. a The protein amount of LC3-II was determined by western blot. β-actin was used as a loading control. N = 7. b Changes in cell viability were analyzed by the MTT assay. N = 3. c The protein levels of occludin were detected by western blot. The bands were normalized by β-actin levels. N = 3. d In vitro FITC-dextran assay and e TEER measurement were conducted to examine cellular permeability. N = 4. Data are presented as the mean ± SEM. *p < 0.05 and **p < 0.01 vs. control; ^#p < 0.05 and ^##p < 0.01 vs. OGD without 3-MA