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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Autophagy-mediated occludin degradation contributes to blood–brain barrier disruption during ischemia in bEnd.3 brain endothelial cells and rat ischemic stroke models

Fig. 2

Activation of autophagy in OGD-exposed brain endothelial cells. The brain endothelial cell line bEnd.3 was exposed to OGD for 18 h. The protein levels of a LC3-II and b p62/SQSTM1 were determined by western blot. Protein levels were normalized to β-actin. N = 3. c Intracellular localization of LC3 and LAMP-1 was examined in OGD-exposed bEnd.3 cells using double immunostaining. Representative images are shown. Scale bar: 50 μm, scale bar in the magnified images: 20 μm. White arrows show the co-localization of LC3 and LAMP1. Pearson’s Correlation Coefficient were calculated using JACoP. Data are presented as the mean ± SEM and analyzed by the Student’s t-test. *p < 0.05 vs. control

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