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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Autophagy-mediated occludin degradation contributes to blood–brain barrier disruption during ischemia in bEnd.3 brain endothelial cells and rat ischemic stroke models

Fig. 1

Changes on cell viability, permeability, and occludin in brain endothelial cells following exposure to oxygen–glucose deprivation (OGD). The brain endothelial cell line bEnd.3 was exposed to OGD for 6, 12 or 18 h. a Cell viability was examined by the MTT assay. N = 3–4. b Functional changes in cellular permeability were analyzed by an in vitro FITC-dextran permeability assay (N = 5) and c transendothelial electrical resistance (TEER) measurements (N = 3) after 18 h OGD. d The protein level of occludin after 18 h OGD was determined by western blot analysis. Relative occludin levels were normalized to β-actin. N = 4. e 18 h OGD-exposed bEnd.3 cells were stained with an antibody to occludin and visualized by immunofluorescence in confocal microscope. Representative images are shown. Scale bar: 50 μm. Data were expressed as the mean ± SEM and analyzed by the Student’s t-test. White arrows show occludin distribution. *p < 0.05 and **p < 0.01 vs. the control group without OGD

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