Fig. 3
Hierarchical clustering of mRNA expression x-fold data normalized to the mean value (from n = 8 from four independent experiments) with of each single target from hCMEC/D3 cells cultivated for 5 days in EBM-2 medium with 5% FCS, subjected to 24 h serum reduction from 5 to 0.25% FCS and were cultivated on day 6 for 5 h under normoxic conditions in DMEM medium with glucose. Norm_Ctrl = 5 h in normoxia in EBM-2 medium on day 6, Norm_Gluc = 5 h in normoxia in DMEM medium containing glucose, OGD = 5 h in DMEM glucose-free medium in the hypoxia chamber at 1% O2, 5% 6d = cells remained in medium supplemented with 5% FCS from day 5 to day 6, 0.25% 6d = cells underwent serum reduction from 5 to 0.25% FCS in the according medium, HC = addition of 100 nM hydrocortisone from day 5 to day 6. The colour code legend is formatted in log-scale