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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Human CD4+ T cell subsets differ in their abilities to cross endothelial and epithelial brain barriers in vitro

Fig. 4

Molecular mechanisms mediating CD4+ T-cell migration across HIBCPP from choroid plexus stroma side to CSF side. Percentage of transmigrated T-cells from peripheral blood of healthy donors B and C across 16 h pro-inflammatory cytokine-stimulated (1 ng/mL TNF-α + 20 IU/mL IFN-γ) inverted HIBCPP cells monolayer pretreated with either anti-human CD99 blocking antibody (20 μg/mL) (a), anti-human ICAM-1 blocking antibody (10 μg/mL) (b) or isotype control antibody are shown. CD4+ T-cells (Th1, Th1*, Th2 and Th17) were allowed to migrate across inverted HIBCPP cells monolayer for 8 h and migrated cells were collected and counted. Results are standardized to isotype control (100%). Data are shown as the mean on superimposed scatter dot plot of 2 independent experiments each performed in triplicates. Statistical analysis: two-way ANOVA followed by Tukey’s multiple comparison test (p < 0.05*, p < 0.001***, p < 0.0001****). Cells used for the representation of the endothelium, the epithelium (HIBCPP cells) and T cells are adapted from Servier Medical Art (http://smart.servier.com/), licensed under a Creative Common Attribution 3.0 Generic License

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