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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Human CD4+ T cell subsets differ in their abilities to cross endothelial and epithelial brain barriers in vitro

Fig. 2

Barrier characteristics of BLECs and HIBCPP cells. a The time-dependent progression of the trans-endothelial or epithelial electrical resistance (TEER) of BLECs and HIBCPP cell monolayers were measured by Volt-Ohm-Meter. BLECs were seeded onto 0.4 μm pore size Costar Transwell® filters and HIBCPP cells onto 5 μm pore size Millicell® filters over a period of 6 days. Solid lines represent mean and error bars show ± SD. Data are representative data from at least 3 experiments each performed in triplicates. b Permeability for 0.45 kDa Lucifer Yellow (LY): BLECs were cultured onto 3 μm pore size Coster Transwell® filters for 7 days as a monoculture and then co-culture with pericyte for 6 days and permeability was measured at day 13. HIBCPP cell were cultured onto 5 μm pore size Millicell® filters and permeability was measured at day 6. Bars show the mean permeability coefficients Pe ± SD of diffused tracer across the BLECs or HIBCPP cell monolayer. Data are Representative data from at least 3 independent experiments with three filters per conditions. Statistical analysis: Student’s t-test p < 0.05*

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