Fig. 6

Comparison of the expression of ion transport regulators OSR1 and SPAK in Ncbe wt and Ncbe ko mouse CP. a Scatter plot illustrating the difference in mean CP OSR1 and SPAK expression between Ncbe wt and Ncbe ko mice, as assessed by comparative mass spectrometry (*p < 0.05, X: Failed FDR of 1%, n = 5). Mean values are normalized to control (Ncbe wt) and indicated by horizontal bars. Triangles indicate data points from Ncbe wt CP, whereas circles represent data from Ncbe ko CP. b Immunofluorescence micrograph showing the cellular distribution of pSPAK/pOSR1 (green) at high magnification of the CP from an Ncbe wt mouse. c A similar micrograph of pSPAK/pOSR1 staining in the CP from an Ncbe ko mouse. The fluorescence images are overlaid onto the corresponding differential interference contrast (DIC) images. Arrows indicate the luminal plasma membrane, while arrowheads indicate the basolateral membrane labyrinth. d, e Immunoblot analysis of the pSPAK/pOSR1 abundance in the CP from Ncbe wt and Ncbe ko mice with two antibodies (“M” and “S”, respectively). Sp indicates the expected migration of pSPAK, whereas O indicates the expected pOSR1 size. f Scatter plot comparing relative changes in pOSR1 and pSPAK abundances obtained by immunoblotting (IB) with the two antibodies (*p < 0.05, n = 5). Mean values are normalized to control (Ncbe wt) and indicated by horizontal bars. Triangles indicate data points from Ncbe wt CP, whereas circles represent data from Ncbe ko CP