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Table 2 Percentage of variation of the different biomarkers measured according to the different volumes of blood spiked into the CSF (expressed as percentage) when compared with the non-spiked CSF samples

From: Effect of blood contamination of cerebrospinal fluid on amino acids, biogenic amines, pterins and vitamins

Biomarkers
(% blood contamination)
A) RBC lysedB) RBC removed
20%10%5%2.5%20%10%5%2.5%
Albumin2724137266732328771379669318
Taurine39319411248189914514
Aspartate14048424012524225< 10< 10
Threonine70432114693814< 10
Serine451813< 102215< 10< 10
Glutamate611394205107572716< 10
Glutamine14< 10< 10< 10< 10< 10< 10< 10
Glycine22911461431146021< 10
Alanine174954623154723011
Citrulline1515622< 101124220< 10
Valine138794023161833613
Methionine141783820151733911
Isoleucine1256834191366929< 10
Leucine1136227171236530< 10
Tyrosine84432011874119< 10
Phenylalanine63381811663918< 10
Ornithine346256154107864114< 10
Lysine1136631161005022< 10
Hystidine934418< 10804218< 10
Arginine− 47− 56− 51− 395127< 10< 10
5-HIAA− 42− 37− 41− 44< 10< 10< 10< 10
HVA− 22− 11< 10< 10< 10< 10< 10< 10
Biopterin< 10< 10< 10< 10< 10< 10< 10< 10
Neopterin12< 10< 10< 10< 10< 10< 10< 10
Thiamine198154175169135683727
5-MTHF74523319< 10< 10< 10< 10
PLP27015710260157804529
Hemoglobin (g/dL)3.021.550.760.37    
TDP (nmol/L)2514114.5    
  1. Data are expressed as the mean value of the 2 CSF pools from the 2 conditions: A) spiked CSF frozen containing lysed-RBC cells (left columns), and B) spiked CSF with RBC removed by centrifugation prior freezing (right columns). A percentage of variation below 10% (comparing spiked CSF samples to the non-spiked CSF samples) was considered as the cut-off value for a proper interpretation of the results, considering the coefficient of variation for every biomarker measured when procedures were standardized. Haemoglobin and TDP values are expressed in g/dL and nmol/L, respectively since their values in condition B were not detectable. A total of 20 CSF aliquots coming from 50 CSF samples were analysed (Additional file 2: Figure S1)