Fig. 3
From: Claudin-12 is not required for blood–brain barrier tight junction function
Claudin-12 is expressed by different cell types in the CNS. Immunofluorescence staining of frozen brain sections from WT and claudin-12lacZ/lacZ C57BL/6J mice, for β-galactosidase (red) and endothelial cells (PECAM-1), pericytes (PDGFRβ), astrocytes (GFAP) and neurons (NeuN) (green), in the brain cortex. Nuclei are stained with DAPI (blue). Boxed areas are shown in higher magnification. β-gal stands for β-galactosidase. Scale bars = 10 and 50 μm, as indicated. Two independent series of stainings were performed