Fig. 1
From: Claudin-12 is not required for blood–brain barrier tight junction function
Claudin-12 targeting strategy in C57BL/6J mice. a Schematic representation of the knock-out strategy. The open reading frame of claudin-12 is located in exon 5 and is targeted by a BAC-based targeting vector that contains an expression and neomycin-selection cassettes. In the targeted allele, a deletion of 760 bps is present, which removes the entire coding sequence. Arrows indicate LoxP sites surrounding the selection cassette that are targeted by Cre recombinase. b Relative expression of different regions of the claudin-12 gene in heart muscle samples isolated from WT and claudin-12lacZ/lacZ C57BL/6J mice was assessed by qRT-PCR. For each region of the gene, technical triplicates in three independent experiments were measured. Relative quantification is represented by the 2−ΔΔCt value. Cldn12 claudin-12, UTR untranslatable region, ORF open reading frame, n.d. not detectable