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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood–brain barrier model

Fig. 3

Glutamate uptake compared between hiPSC astroglia differentiated on human, recombinant laminin-521 (LN521) and murine laminin (L2020). a Astroglia uptake of glutamate in the presence and absence of the EAAT1 inhibitor UCPH101 (UCPH). Astroglia differentiated on both LN521 and L2020 have glutamate uptake, which is inhibited by UCPH 101. C1 and AF astroglia differentiated on LN521 show a larger reduction in glutamate uptake when treated with the inhibitor UCPH. b Representative immunocytochemistry images show that both astroglia differentiated on LN521 and L2020 express EAAT1. c Image analysis of EAAT1 expression showed that the percentage of cells expressing EAAT1 was higher in astroglia differentiated on LN521 compared to L2020. d mRNA analysis of EAAT1 showed that astroglia differentiated on LN521 had higher expression of EAAT1 mRNA, compared to astroglia differentiated on L2020. Neuro epithelial stem cells (NES) included as reference. Significance indicated by *p < 0.05, **p < 0.01, ***p < 0.001

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