Fig. 4
Determination of a fluorescently labeled dextran ability to cross RA-treated induced pluripotent stem cell (iPSC)-derived brain microvascular endothelial cells (BMECs) from the apical to the basolateral chamber in the two-compartment co-culture model. BMECs in monoculture (MC) or in co-culture for 48 h with either pericytes (P), neurons and astrocytes (NA), or pericytes, neurons, and astrocytes (PNA) were presented with a fluorescently tagged dextran for 2 h. Fluorescently-tagged dextran was measured within the BMEC population (a; accumulation) and from the bottom chamber (b; transcytosis) at both 37 °C and 4 °C. Raw fluorescence units are normalized to monoculture BMECs. Statistical significance was determined with ANOVA. Values are mean ± SD of three independent differentiations. *p < 0.05 versus MC