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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: An isogenic neurovascular unit model comprised of human induced pluripotent stem cell-derived brain microvascular endothelial cells, pericytes, astrocytes, and neurons

Fig. 2

Analysis of tight junction continuity following co-culture. Localization of tight junction proteins, occludin, claudin-5, and ZO-1 were investigated in RA treated iPSC-derived BMECs following 48 h of co-culture with IMR90-iPSC-derived pericytes (P), neurons and astrocytes (1:3; NA) or pericytes (initial 24 h) followed by 24 h of neurons and astrocytes (1:3; PNA). a Immunocytochemistry of occludin revealed numerous discontinuous junctions (white arrows) in BMECs in monoculture compared to co-culture. Scale bar = 100 μm. b Quantification of the tight junction localization in BMECs in monoculture and co-culture conditions were calculated by the area of each image having immune-reactive occludin, claudin-5, or ZO1, resulting in area fraction index. Statistical significance calculated using ANOVA. Values are mean ± SD of three independent differentiations. *p < 0.05 versus MC, #p < 0.05 versus P, and †p < 0.05 versus the NA group

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