Fig. 4

Summary of grouped characterization of barrier function and transport systems for healthy lines (BC1, WT2, and iPS12) and disease lines (JH033, SODA4V, SCNA1, SCNAT, AD6, AD10, HD50, HD71). a Schematic illustration of barrier function. b Schematic illustration of transport systems. c Summary of impairment in TEER, P(LY), P(glucose), and efflux ratio for disease lines with respect to grouped healthy controls. TEER measurements for healthy lines (N = 13 independent differentiations), and disease lines (N = 30 independent differentiations). Lucifer yellow permeability for healthy lines (N = 9, 3 independent differentiations), and disease lines (N = 24 independent differentiations). Glucose permeability for healthy lines (N = 10 independent differentiations), and disease lines (N = 26 independent differentiations). Efflux ratio for rhodamine 123 permeability for healthy lines (N = 14 independent differentiations), and disease lines (N = 33 independent differentiations). Statistical analysis was performed using nested ANOVA comparing to the three healthy controls. *p < 0.05