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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: The role of mutations associated with familial neurodegenerative disorders on blood–brain barrier function in an iPSC model

Fig. 1

Protein and gene expression in dhBMECs from healthy individuals and individuals with NDD patients. a Protein expression from Western blots for claudin-5, occludin, P-gp, and ZO-1 for all 11 cell lines. Data were obtained from two technical replicates for each of three independent differentiations (N = 3). Results were normalized by first correcting for protein concentration based on β-actin loading control, and then normalized to the first technical replicate of BC1 run on each gel. Selected bands are provided in Additional file 1: Figure S2. b mRNA expression assessed using qRT-PCR for CLDN5, OCLN, ABCB1 (P-gp), and SLC2A1 (GLUT1) for all 11 cell lines. Expression levels are normalized to the mean expression level for that gene in the BC1 dhBMEC line after normalization to the housekeeping genes BACT and GPADH. Data were obtained from three technical replicates for each of three differentiations (N = 3). c Percent increase in amount of reactive oxygen species in response to stress induced by exposure to 5 and 50 mM menadione for 30 and 60 min. Data were obtained from two technical replicates for each of three differentiations (N = 3). Error bars represent mean ± SE. Statistical analysis was performed using nested ANOVA comparing to the three healthy controls. *p < 0.05

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