Table 1 List qRT–PCR mouse primer sequences
From: Human ES-derived MSCs correct TNF-α-mediated alterations in a blood–brain barrier model
Gene | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
RPL-19 | CGCTGCGGGAAAAAGAAG | CTGATCTGCTGACG GAGTTG |
CLN-5 | TGCCGCGAACAGTTCCTAC | CCAGCTGCCCTTTCAGGTTA |
ZO-1 | CTCGGAAAAATGAAGAATATGGTC | CACCATCTCTTGCTGCCAAA |
Occludin | GGACTGGGTCAGGGAATATCC | GCAGACCTGCATCAAAATTTCTC |
VE-cadherin | CACTGCTTTGGGAGCCTTC | GGGGCAGCGATTCATTTTTCT |
ICAM-1 | GGTGACTGAGGAGTTCGACAGAA | ACCGGAGCTGAAAAGTTGTAGACT |
VCAM-1 | GTGACTCCATGGCCCTCACT | CGTCCTCACCTTCGCGTTTA |
CCL2 | GGCTCAGCCAGATGCAGTTAA | CC GCCTACTCATTGGG TCA |
CXCL12 | GCTCCTCGACAGATGCCTTG | GACCCTGGCACTGAACTGGA |