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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Human ES-derived MSCs correct TNF-α-mediated alterations in a blood–brain barrier model

Fig. 1

TNF-α-induced changes in barrier properties in BMEC. a BMEC monolayer permeability. BMECs were plated on 24-well Transwell inserts, allowed to achieve confluence, and then (±) exposed to 10 ng/mL TNF-α added to both the bottom and top chamber for 24 h at 37 °C. After this time, hES-MSCs were added to either the bottom (B) or top (T) chamber for an additional 24 h. Subsequently, 100 μg/ml fluorescein dextran was added to the top chamber. Samples (50 μl) were removed from the bottom chamber at 2 h and analyzed by plate reader. b BMEC monolayer TEER. BMECs were grown to confluence on Transwell filter inserts. Following TNF-α and hES-MSC treatments, TEER measurements were performed. Changes in permeability and TEER following different treatments were reported as x-fold change of control value. Data are presented as mean ± SE. Each experiment consisted of 3 replicates (derived from a single preparation of BMECs) repeated 3 times (each time from a different BMEC preparation), for a total N = 9 samples per group. *p < 0.05 compared with control group, #p < 0.05 compared with TNF-α treated group. For purposes of comparison to the literature, absolute mean values of controls for permeability and TEER were 1.2 × 10−5 cm/min and 256 Ω cm2, respectively

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