Fig. 4

Confocal images of direct contact 2D co-culture of dhPCs and dhBMECs. a, c Confocal z-stack and cross-section images 7 days after seeding dhPCs on dhBMECs (a) and dhBMECs on dhPCs (c). In a and c XY slices are shown at the height of the dhBMEC layer (top), and at the height of the dhPC layer (bottom). The orange lines denote the plane of the YZ cross section. The green and red lines segments in the sections correspond to the z-positions of the top and bottom XY slices (outlined in green and red, respectively). b, d Quantification of the z-position of the nuclei of dhBMECs and dhPCs relative to the glass surface after seeding dhPCs on dhBMECs (b) and dhBMECs on dhPCs (d). Data represent mean ± SD. For b and d at least 44 pairs of cells from at least 3 fields of view from at least 2 wells per seeding were quantified. e Collagen IV stain following seeding dhBMECs on dhPCs. The cross section is magnified (orange outline). White arrows in the cross-section indicate collagen IV above and below dhPCs. f Laminin stain following seeding dhBMECs on dhPCs. White arrows indicate laminin above and below dhPCs