Fig. 3

Barrier function of dhBMEC monolayers on transwells in contact co-culture with dhPCs. a–c Time dependence of TEER values for the experimental configurations shown in the schematic illustrations. a dhPCs on dhBMECs (P-on-E). b dhBMECs on dhPCs (E-on-P). c A mixture of dhBMECs and dhPCs. (E + P). In each configuration experiments were performed at PC:EC ratios of 1:50 (low) and 1:5 (high). TEER values are also shown for dhBMEC monolayers (EC) and dhPCs only (PC). TEER values have been normalized to the peak of the control (no dhPCs), such that each biological replicate of the control reaches a maximum relative TEER of 1.0 at its highest point. d Peak TEER achieved by each co-culture condition with low (1:50) PC:EC ratio. e Peak TEER at high (1:5) PC:EC ratio. Data represent mean ± SEM, *P < 0.05, **P < 0.01. All experiments were performed with three biological replicates (independent dhBMEC differentiations) and at least two technical replicates (transwell experiments for each differentiation)