Fig. 1
Differentiation and characterization of dhBMECs and dhPCs. a Differentiation scheme for dhBMECs. b Immunofluorescence staining of dhBMEC monolayers for proteins associated with tight (claudin-5, occludin, and ZO-1) and adherens (VECad) junctions, performed 48 h after dhBMEC subculture, displayed alone for clarity (top row), and with cell nuclei and f-actin labeled by DAPI and phalloidin, respectively (bottom row). c Differentiation scheme for dhPCs. d Immunofluorescence staining of dhPCs for established pericyte and mural cell markers (PDGFRβ, NG2, αSMA, and calponin) displayed alone for clarity (top row), and with cell nuclei and f-actin labeled by DAPI and phalloidin, respectively (bottom row). e Representative live-cell flow cytometry histograms of dhPCs for pericyte and mesenchymal surface markers (PDGFRβ, CD73, CD105, and absence of VECad). The percentages listed on each histogram are the mean ± SD of at least three biological replicates