Fig. 7From: Disruption of the hippocampal and hypothalamic blood–brain barrier in a diet-induced obese model of type II diabetes: prevention and treatment by the mitochondrial carbonic anhydrase inhibitor, topiramateEffect of diet and topiramate on tight junction protein expression using immunoblot. Whole brain lysate was used to examine expression of the tight junction proteins, claudin-5, claudin-12, and ZO-1, and caveolin-1, the major structural protein of caveolae (a). HF-diet consumption led to a significant decrease in claudin-12 and ZO-1 expression which was attenuated with TPM treatment for claudin-12 (b). There were no significant changes in calveolin-1 or claudin-5. Values are expressed as mean ± SD and relative to the β-actin loading control. Significance was determined by a one-way analysis of variance followed by Newman–Keuls post-test between HF vs. LF, LF vs. LF TPM, and HF vs. HF TPM. *p < 0.05; #p < 0.001Back to article page