Tunneling nanotubes evoke pericyte/endothelial communication during normal and tumoral angiogenesis

Table 1 List of primary and secondary antibodies

Primary antibodies	Host IgG	Dilution	Concentration	Producer	Cat. no.
Fibronectin	Rabbit IgG	1:100	5 mg/ml	Dako	A0245
CD31	Mouse IgG_1k	1:10	0.205 mg/ml	Dako	M0823
CD105	Rabbit IgG	Prediluted	NA	Abcam	AB27422
Glut-1	Goat IgG	1:100	0.2 mg/ml	Santa Cruz	sc-1605
CD146	Mouse IgG₁	1:50	0.123 mg/ml	Abcam	AB49492
NG2/CSPG4 D2	Rabbit IgG	1:50	NA	W.B. Stallcup	–
Col type IV	Mouse IgG_1k	1:100	0.5 mg/ml	Dako	M0785
Col type IV	Rabbit IgG	1:100	1 mg/ml	Acris	R1041
Col type VI	Rabbit IgG	1:100	1 m/ml	Abcam	AB6588
GFAP	Mouse IgG₁	1:100	0.1 mg/ml	Monosan	MONX10749
NG2/CSPG4	Mouse IgG	1:200	0.5 mg/ml	Thermo Fisher	14-6504-80

Secondary antibodies	Dilution	Concentration	Producer	Cat. no.
Biotinylated goat-anti rabbit	1:400	1.5 mg/ml	Vector	BA-1000
Streptavidin-Alexa 488	1:300	2 mg/ml	Thermo Fisher	S-11223
Streptavidin-Alexa 555	1:300	2 mg/ml	Thermo Fisher	S-21381
Goat anti mouse IgG Alexa 488	1:300	2 mg/ml	Thermo Fisher	A11001
Goat anti mouse IgG₁ Alexa 555	1:300	2 mg/ml	Thermo Fisher	A21127
Goat anti mouse IgG₁ Alexa 633	1:300	2 mg/ml	Thermo Fisher	A21126
Goat anti-rabbit Alexa 488	1:300	2 mg/ml	Thermo Fisher	A11070
Donkey anti-goat Alexa 488	1:300	2 mg/ml	Thermo Fisher	A11055
Goat anti-rabbit Alexa 555	1:300	2 mg/ml	Thermo Fisher	A32732

ISSN: 2045-8118