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Fig. 8 | Fluids and Barriers of the CNS

Fig. 8

From: Tunneling nanotubes evoke pericyte/endothelial communication during normal and tumoral angiogenesis

Fig. 8

Human brain vascular pericytes (HBVP) TNTs in vitro. a, d Representative TNT networking among HBVP cultured on Matrigel layer. b, e Filaments of actin contained in HBVP-derived TNTs stained with Phalloidin TRITC-conjugated dye. c, f NG2/CSPG4 immunostaining of pericytes (green), pretreated with Phalloidin TRITC-conjugated dye (c, red), or with lipophilic DiI cell tracker (f, red), shows long NG2+ TNTs (arrows). Nuclear counterstaining DAPI (b, c, e, f). Scale bars a, b, d, e 50 µm; c, f 25 µm

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