Fig. 6From: Tunneling nanotubes evoke pericyte/endothelial communication during normal and tumoral angiogenesisRepresentative confocal microscopy images of pericyte TNT involved in vessel growth and sprouting in the developing human telencephalon at 22 wg (a–c) and 18 wg (d–f). a–c CD105/CD146 and d–f NG2/CD146 double immunostainings. a–c Comparison of the merged image (a) with single channels (b, c) allows a ‘complete’ vessel sprout to be identified, formed by a CD146+ escorting pericyte (PC in a and b) and a CD105+ endothelial cell (arrowhead in a, c) and a CD146+, pericyte-like sprout (star in a and b), that does not stain positive for CD105, connected by a CD146+ pericyte-like TNT (arrow in a and b); the encircled area in b corresponds to an overexposed laser confocal microscope setting. d–f NG2 colocalized with CD146 on a leading pericyte (PC) and on its short TNT (arrow). Nuclear counterstaining TOPRO-3. Scale bars a–f 25 µmBack to article page