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Fig. 6 | Fluids and Barriers of the CNS

Fig. 6

From: Tunneling nanotubes evoke pericyte/endothelial communication during normal and tumoral angiogenesis

Fig. 6

Representative confocal microscopy images of pericyte TNT involved in vessel growth and sprouting in the developing human telencephalon at 22 wg (a–c) and 18 wg (d–f). a–c CD105/CD146 and d–f NG2/CD146 double immunostainings. a–c Comparison of the merged image (a) with single channels (b, c) allows a ‘complete’ vessel sprout to be identified, formed by a CD146+ escorting pericyte (PC in a and b) and a CD105+ endothelial cell (arrowhead in a, c) and a CD146+, pericyte-like sprout (star in a and b), that does not stain positive for CD105, connected by a CD146+ pericyte-like TNT (arrow in a and b); the encircled area in b corresponds to an overexposed laser confocal microscope setting. d–f NG2 colocalized with CD146 on a leading pericyte (PC) and on its short TNT (arrow). Nuclear counterstaining TOPRO-3. Scale bars a–f 25 µm

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