Fig. 5

Representative confocal microscopy images of NG2/CD146 immunostained pericyte TNTs in the developing human telencephalon at 22 wg. a A very thin, double stained TNT, revealed by the overexposed laser confocal microscope setting (encircled area), originates from an NG2⁺/CD146⁺ pericyte (PC). b, c NG2 and CD146 extensively colocalized along the entire extension of pericytes TNTs (arrows), although on the TNT distant ends CD146 staining prevails. d An NG2⁺/CD146⁺ larger TNT conduit (arrow) appears to connect the tip of a growing microvessel (V), escorted by a leading pericyte (PC), with a recipient vessel (V); note a nucleus (arrowhead) that apparently fills the TNT end (see also Additional file 6). Nuclear counterstaining TOPRO-3. PC, pericyte. Scale bars a, d 10 µm; b, c 25 µm