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Table 2 Expression of GLUT1 at the blood–brain barrier

From: Elimination of substances from the brain parenchyma: efflux via perivascular pathways and via the blood–brain barrier

Source Methoda Species Expression/pmol mg−1
Luminal Totalb Abluminal
  Relative to microvessel protein
Dick et al. [303] Cytochalasin B binding Rat, pig   69–80  
Kalaria et al. [571] Cytochalasin B binding Human   42  
Farrell and Pardridge [572] Immunogold e.m Rat 12%c 100%c 48%c
Cornford et al. [573] Immunogold e.m Human 48% 100% 18%
Vannucci et al. [574] Cytochalasin B binding Rat   40–125  
Kamiie et al. [182] Proteomics Mouse   90  
Uchida et al. [184] Proteomics Human   139  
Shawahna et al. [176] Proteomics Human   78.5  
Hoshi et al. [185] Lysate digestion proteomics Rat   84–98  
  Relative to membrane protein
Simpson et al. [575] Fractionation, cytochalasin B binding Bovine 620   280
Kubo et al. [58] Fractionation, proteomics Porcine 79%   21%
Zhang et al. [188] Proteomics Porcine   300  
  1. aFractionation = fractionation of isolated plasma membranes
  2. bFor immunogold detection, values are percentages of the immunogold particles where the total includes cytoplasmic
  3. cAntigen for the antibody used by Farrell and Pardridge appears to be partially masked for GLUT1 most markedly in the luminal membrane in bovine endothelial cells [575]