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Table 2 Expression of GLUT1 at the blood–brain barrier

From: Elimination of substances from the brain parenchyma: efflux via perivascular pathways and via the blood–brain barrier

Source

Methoda

Species

Expression/pmol mg−1

Luminal

Totalb

Abluminal

 

Relative to microvessel protein

Dick et al. [303]

Cytochalasin B binding

Rat, pig

 

69–80

 

Kalaria et al. [571]

Cytochalasin B binding

Human

 

42

 

Farrell and Pardridge [572]

Immunogold e.m

Rat

12%c

100%c

48%c

Cornford et al. [573]

Immunogold e.m

Human

48%

100%

18%

Vannucci et al. [574]

Cytochalasin B binding

Rat

 

40–125

 

Kamiie et al. [182]

Proteomics

Mouse

 

90

 

Uchida et al. [184]

Proteomics

Human

 

139

 

Shawahna et al. [176]

Proteomics

Human

 

78.5

 

Hoshi et al. [185]

Lysate digestion proteomics

Rat

 

84–98

 
 

Relative to membrane protein

Simpson et al. [575]

Fractionation, cytochalasin B binding

Bovine

620

 

280

Kubo et al. [58]

Fractionation, proteomics

Porcine

79%

 

21%

Zhang et al. [188]

Proteomics

Porcine

 

300

 
  1. aFractionation = fractionation of isolated plasma membranes
  2. bFor immunogold detection, values are percentages of the immunogold particles where the total includes cytoplasmic
  3. cAntigen for the antibody used by Farrell and Pardridge appears to be partially masked for GLUT1 most markedly in the luminal membrane in bovine endothelial cells [575]