Fig. 3
From: Blood–brain and blood–cerebrospinal fluid barrier permeability in spontaneously hypertensive rats
Visualisation and quantification of the rat brain microvasculature. The vascular endothelium was visualised by a DyLight®594-labelled L. esculentum lectin (red), and cell nuclei by DAPI staining (blue). a Overview of a coronal rat brain section indicating the stereotaxic coordinates and 6 different brain regions used to quantify a number of vascular parameters. b and c Representative images of the lectin staining used for the analysis of vascular parameters in the CA3 and VM respectively. d Volume fraction of capillaries did not differ between WKY (n = 10) and SHR (n = 10) in six different brain regions. Values are mean ± SEM (repeated measures ANOVA, Bonferroni’s post hoc tests). cc, corpus callosum; Cx, cerebral cortex; CA3, field CA3 of the hippocampus; VM, ventromedial thalamic nucleus; VMH, ventromedial hypothalamic nucleus; Pir, piriform cortex. Scale bar in a represents 1 mm, and 100 µm in b and c