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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: A perfused human blood–brain barrier on-a-chip for high-throughput assessment of barrier function and antibody transport

Fig. 1

Perfused microvessels of TY10 brain endothelial cells in the two-lane OrganoPlate. a Picture shows the two-lane OrganoPlate. The plate combines a 384-well microtiter plate on the top with microfluidic channels on the bottom that make up 96 tissue culture chips. b Zoom-in of the bottom of the two-lane OrganoPlate, showing the tissue culture chips that consist of two channels: a gel channel and a medium channel. c 3D artist impression of the center of a chip. ECM gel is added to the gel channel and a phaseguide (phg) prevents it from flowing into the adjacent medium channel. After gelation of the ECM gel, TY10 cells are added to the medium channel and a TY10 microvessel forms. The microvessel has a lumen at its apical side that is perfused. d Maximum projection image of a TY10 microvessel stained with ActinRed. Scale bar is 50 µm. e 3D reconstruction of a confocal z-stack showing a perfused TY10 microvessel. Inlay shows a vertical cross section, depicting the lumen, the phaseguide, and the ECM gel channel. f–h Immunostaining of a TY10 microvessel for VE-cadherin, PECAM-1, and claudin-5. Scale bar is 50 µm

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Fluids and Barriers of the CNS

ISSN: 2045-8118