Accurate, strong, and stable reporting of choroid plexus epithelial cells in transgenic mice using a human transthyretin BAC

Table 1 PCR primers used in the study

Category	Primer pair	Direction	Sequence (5′ to 3′)	Amplicon (bp)
genotyping	tdTom internal	Forward	CACCATCGTGGAACAGTACG	142
	tdTom internal	Reverse	GCGCATGAACTCTTTGATGA	142
	BAC 100 kb 5′ #1	Forward	TTAGGATTCAGGTGGCCTTG	148
	BAC 100 kb 5′ #1	Reverse	TGCACATCCTTGGCAATAAA	148
	BAC 100 kb 5′ #2	Forward	AATGAAGAGGCTGCCAAAGA	192
	BAC 100 kb 5′ #2	Reverse	AGTGGATCCCACGACAGTTC	192
	BAC 60 kb 5′	Forward	AAGCCCAAGATCAAAGCAGA	202
	BAC 60 kb 5′	Reverse	CTCACGTGCTGAAATCCTGA	202
	BAC 40 kb 3′	Forward	TCAGCAGCTTCCTGCTACAC	500
	BAC 40 kb 3′	Reverse	GCTAGACAGGTACCCAGGGA	500
	luc2 internal	Forward	ACAGAAACAACCAGCGCCATTCTG	590
	luc2 internal	Reverse	TCCAACTTGCCGGTCAGTCCTTTA	590
	JAX control	Forward	CTAGGCCACAGAATTGAAAGATCT	324
	JAX control	Reverse	GTAGGTGGAAATTCTAGCATCATCC	324
RT-qPCR	tdTomato	Forward	ATCGTGGAACAGTACGAGCG	133
	tdTomato	Reverse	TGAACTCTTTGATGACGGCCA	133
	transthyretin	Forward	ATCGTACTGGAAGACACTTGGC	131
	transthyretin	Reverse	CCGTGGTGCTGTAGGAGTAT	131
	18S ribosomal	Forward	CGGCTACCACATCCAAGGAA	187
	18S ribosomal	Reverse	GCTGGAATTACCGCGGCT	187
	cyclophilin A	Forward	GAGCTGTTTGCAGACAAAGTTC	125
	cyclophilin A	Reverse	CCCTGGCACATGAATCCTGG	125

ISSN: 2045-8118