Fig. 5

TdTomato continues to be expressed in postnatal choroid plexus of BAC transgenic mice. A Show paired images of transmitted light and tdTomato fluorescence in wet mounts of dissected lateral ventricle ChP from post-weanling mice from three founder lines. Whereas transgenic ChP (+) fluoresce brightly, ChP from wild-type siblings (−) do not. B Show immunofluorescence in cryostat sections from a P3 brain. TdTomato was detected in all four ventricles and was co-localized with anion exchanger 2 (AE2, right). C–E Shows immunofluorescence in microtome sections of brains from 314-day-old mice, verifying the long-term expression of the reporter in CPECs of the lateral (C), third (D), and fourth (E) ventricle. The selective localization of tdTomato in CPECs is confirmed by associated staining with antibodies to TTR. The location of nuclei is indicated by DAPI staining