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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Zika virus crosses an in vitro human blood brain barrier model

Fig. 1

Direct ZIKV infection and AXL expression in i-NP and i-Ns. ad ZIKV infection was detected in the cytoplasm of b i-NPs and d i-Ns by immunofluorescence staining 72 h after ZIKV infection (MOI = 4). The nuclei were stained with Hoechst dye (blue) and the virus was detected using anti-flavivirus group antibody (FV; green). i-Ns were additionally stained with the anti-MAP2 (red) antibody. Scale bar = 10 µm. e Fold difference of ZIKV (ZV) infection in i-NP, relative to i-Ns, based on cell counts of FV positive cells (mean ± SD). Cell counts are from two independent experiments, six fields counted, with at least 80 cells/field. (****p < 0.0001, Student’s T test). f A representative Western blot of putative ZIKV receptor AXL expression in i-NPs and i-Ns detected by an anti-AXL antibody. β-ACTIN was used as an internal loading control

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