Fig. 3From: Modeling and rescue of defective blood–brain barrier function of induced brain microvascular endothelial cells from childhood cerebral adrenoleukodystrophy patientsccALD-iBMECs accumulate more lipid droplets than WT-iBMECs. a Comparison of transmission electron micrographs of WT1- and ccALD3-iBMECs show increased lipid droplet accumulation in ccALD-iBMECs. Lipid droplets outlined in red. b Representative images of Oil-Red-O stained WT3 and ccALD1-iBMECs. Raw images on left and masked images on right. c Quantification of intensity and number of red pixels in images of Oil-Red-O stained iBMECs indicate increased lipid droplet accumulation in ccALD-iBMECs compared to WT-iBMECs. Oil-Red-O staining images of all iBMEC lines were used for quantification using three biological replicates for each cell line (n = 9)Back to article page