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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Modeling and rescue of defective blood–brain barrier function of induced brain microvascular endothelial cells from childhood cerebral adrenoleukodystrophy patients

Fig. 2

ccALD-iBMECs are functionally distinct from WT-iBMECs. a Trans-endothelial electrical resistance (TEER) is significantly decreased in the ccALD-iBMECs compared to the WT-iBMECs at all experimental time points. Data compiled from three independent experiments with nine biological replicates each (all iBMEC lines used) (n = 27). *p < 0.0001. b Passive transport as measured by sodium fluorescein permeability is slightly increased in the ccALD-iBMECs compared to WT-iBMECs. All iBMEC lines tested with three biological replicates each (n = 9). c Examples of frayed junctions indicated by white arrows on occludin immunolabeled images of WT1- and ccALD3-iBMECs. d Quantification of percent frayed junctions in WT1- and ccALD3-iBMECs indicates that WT-iBMECs have fewer frayed junctions than ccALD-iBMECs. Results of nine biological replicates with five technical replicates each shown (n = 45)

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Fluids and Barriers of the CNS

ISSN: 2045-8118