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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Functional brain-specific microvessels from iPSC-derived human brain microvascular endothelial cells: the role of matrix composition on monolayer formation

Fig. 1

The influence of ECM on monolayer formation. a Cell coverage on varying concentrations of collagen I gels coated with fibronectin and collagen IV at 24, 48, and 72 h following seeding. b Cell coverage on 7 mg mL−1 collagen I gels supplemented with laminin/entactin and coated with fibronectin and collagen IV. c Coverage on 7 mg mL−1 collagen I gel coated with the proteins indicated fn—fibronectin, cnIV—collagen IV, ln—laminin. All coatings result in significantly higher coverage compared to uncoated collagen I gels (P < 0.05), with no difference between different coatings (P > 0.05). d Coverage on 7 mg mL−1 collagen I gel coated with selected proteins (fn—fibronectin, cnIV—collagen IV) and selected heparan sulfate proteoglycans (ag—agrin, pc—perlecan). Bars represent mean ± standard error, * represents p < 0.05, *** represents p < 0.001

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