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Table 2 Effect of Na+- or Cl-replacement, and co-presence of compounds on [3H]p-TA uptake by TR-CSFB3 cells

From: Role of cationic drug-sensitive transport systems at the blood-cerebrospinal fluid barrier in para-tyramine elimination from rat brain

Compounds Concentration (mM) Percentage of control
Na + /Cl -replacement
 Control   100 ± 3
 Na+-free   153 ± 8**
 Cl-free   103 ± 4
Co-presence of compounds
 Control   100 ± 1
 p-TA 10 51.8 ± 3.7**
 Propranolol 10 29.0 ± 4.2**
 Imipramine 10 31.5 ± 2.9**
 Amantadine 10 45.3 ± 2.4**
 Nicotine 10 52.2 ± 3.8**
 Desipramine 10 63.4 ± 1.9**
 Pyrilamine 10 65.4 ± 10.8**
 Verapamil 3.0 69.6 ± 6.7*
 l-Carnitine 10 75.0 ± 5.7
 Norepinephrine 10 96.1 ± 7.3
 PAH 10 110 ± 6
 Serotonin 10 114 ± 5
 Cimetidine 10 115 ± 2
 Tyrosine 10 140 ± 17**
 Choline 10 150 ± 6**
 MPP+ 10 155 ± 19**
 TEA 10 168 ± 13**
 Control (1% DMSO)   100 ± 5
 Pyrimethamine (1% DMSO) 0.2 91.0 ± 7.3
  1. [3H]p-TA uptake by TR-CSFB3 cells (0.15 µCi/well) was performed at 37 °C for 2 min in the absence (control) or presence of Na+ and Cl without (control) or with unlabeled compounds. Each value represents the mean ± S.E.M. (n = 3)
  2. DMSO dimethyl sulfoxide, MPP + 1-methyl-4-phenylpyridinium, PAH p-aminohippurate, TEA tetraethylammonium, p-TA para-tyramine
  3. *p < 0.05 and **p < 0.01, significantly different from control