Skip to main content

Table 2 Effect of Na+- or Cl-replacement, and co-presence of compounds on [3H]p-TA uptake by TR-CSFB3 cells

From: Role of cationic drug-sensitive transport systems at the blood-cerebrospinal fluid barrier in para-tyramine elimination from rat brain

Compounds

Concentration (mM)

Percentage of control

Na + /Cl -replacement

 Control

 

100 ± 3

 Na+-free

 

153 ± 8**

 Cl-free

 

103 ± 4

Co-presence of compounds

 Control

 

100 ± 1

 p-TA

10

51.8 ± 3.7**

 Propranolol

10

29.0 ± 4.2**

 Imipramine

10

31.5 ± 2.9**

 Amantadine

10

45.3 ± 2.4**

 Nicotine

10

52.2 ± 3.8**

 Desipramine

10

63.4 ± 1.9**

 Pyrilamine

10

65.4 ± 10.8**

 Verapamil

3.0

69.6 ± 6.7*

 l-Carnitine

10

75.0 ± 5.7

 Norepinephrine

10

96.1 ± 7.3

 PAH

10

110 ± 6

 Serotonin

10

114 ± 5

 Cimetidine

10

115 ± 2

 Tyrosine

10

140 ± 17**

 Choline

10

150 ± 6**

 MPP+

10

155 ± 19**

 TEA

10

168 ± 13**

 Control (1% DMSO)

 

100 ± 5

 Pyrimethamine (1% DMSO)

0.2

91.0 ± 7.3

  1. [3H]p-TA uptake by TR-CSFB3 cells (0.15 µCi/well) was performed at 37 °C for 2 min in the absence (control) or presence of Na+ and Cl without (control) or with unlabeled compounds. Each value represents the mean ± S.E.M. (n = 3)
  2. DMSO dimethyl sulfoxide, MPP + 1-methyl-4-phenylpyridinium, PAH p-aminohippurate, TEA tetraethylammonium, p-TA para-tyramine
  3. *p < 0.05 and **p < 0.01, significantly different from control