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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Profiling of metalloprotease activities in cerebrospinal fluids of patients with neoplastic meningitis

Fig. 4

Protease activity decreases following intrathecal treatment with liposomal cytarabin. In a 62-year old breast cancer patient with leptomeningeal metastasis, protease activities were analyzed prior and after intrathecal treatment with liposomal cytarabin. Time-lapse fluorimetry output of the same patient with neoplastic meningitis arising from breast cancer previous (NM, black dots) and following treatment of NM with Cytarabin (araC) (NM + AraC, grey dots) for 3 months. Substrate tracking (PEPDab 5, 8, 10, 13 and 14) for 30 min averaged over 3 technical replicates is shown, dashed line indicates the negative control. Cleavage rates were calculated as described previously using a non-linear kinetic model to fit the time-lapse data (a). PrAMA inference results for samples analysed in A, using significance threshold σT = 1.4. Error bars indicate standard deviation of technical triplicates (b). Two-tailed, one-sample t test was used to compare cleavage rates pre and post treatment with araC. Values were not significant (ns, p ≥ 0.05), significant * (p ≤ 0.05) or highly significant ** (p ≤ 0.01)

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