Fig. 1

Analysis of protease activities in the CSF of three patient cohorts. Cleavage signatures in CSF samples were analyzed from 12 control individuals (crtl), 12 patients with neoplastic meningitis (NM) and 12 patients with brain metastases of different primary tumors, but without neoplastic meningitis (w/o NM). Protease activities in real-time mode were determined using PEPDab substrates 5, 8, 10, 13 and 14 at final concentrations of 10 µM for 4 h. Heat map summarizing mean cleavage rates of 3 technical replicates for each clinical samples calculated from time-lapse fluorimetry a. Hierarchical bi-clustering result corresponding to (a), with data mean-centered and variance-normalized by row. CSF cleavage patterns of patients with NM (labeled red) cluster closely together, control individuals and patients with brain metastases w/o NM group together without forming main clusters for each condition (b)