Fig. 9

Protein and gene expression of dhBMECs in confluent monolayers after 40 h at 0, 4, and 12 dyne cm⁻²shear stress. a Relative intensities of protein expression of CLDN-5, LAT-1, and ZO-1 using western blot analysis. Data were obtained from analysis of four different differentiations for CLDN-5 and LAT-1 and three differentiations for ZO-1. Fold changes are reported with respect to static conditions (0 dyne cm⁻²). β-actin was used as a control. Error bars represent SE. Asterisk represents p < 0.05. b Relative gene expression of ABCB1 (P-gp), CDH5 (VE-cad), CLDN5 (claudin-5), OCLN (occludin), SLC2A1 (GLUT-1), and TJP1 (ZO-1) from qPCR. Data were obtained from analysis of three separate differentiations. Fold changes are reported with respect to static conditions (0 dyne cm⁻²). Error bars represent mean ± SE. ACTB and GAPDH were used as the housekeeping genes