Fig. 6
E6-derived BMECs respond to inductive cues from astrocytes and pericytes. a Co-culture of CD12-derived BMECs with astrocytes, pericytes, and a mixture of astrocytes and pericytes achieved maximum TEER values exceeding 4000 Ω × cm2 and maintained TEER above 1000 Ω × cm2 for a minimum of 22 days under all co-culture conditions. Each condition was conducted on triplicate filters with all BMECs purified from a single differentiation. Each filter was measured at three different locations on the filter each day. Values are mean ± standard deviation from these collective nine technical replicates per condition per day. Maximum TEER values achieved on day 8 of subculture were normalized to the TEER of the monoculture control. Statistical significance was calculated using Student’s unpaired t test. b CC3-derived BMECs were co-cultured with a mixture of astrocytes and pericytes, achieving a significant increase in TEER 24 h after barrier induction (p < 10−5, Student’s unpaired t test). Each condition was conducted on triplicate filters with all BMECs purified from a single differentiation. Each filter was measured at three different locations on the filter each day. Values are mean ± standard deviation from these collective nine technical replicates per condition per day. BMECs were subsequently stained for occludin and claudin-5 in both control and co-culture conditions. Scale bars are 50 μm