Fig. 4
E6-derived BMECs and UM-derived BMECs demonstrate equivalent efflux transporter activity. E6-derived BMECs and UM-derived BMECs were incubated with rhodamine 123 (R123) or H2DCFDA in the presence and absence of efflux transporter inhibitors, PSC833 and MK-571, respectively. Fluorescence accumulation within cells incubated with fluorescent substrate in the presence of the desired inhibitor was normalized to fluorescence accumulation in cells incubated with substrate but with no inhibitor. Each condition was performed using triplicate wells, and cell count per condition was calculated as an average from 6 images taken from 1 additional well for each condition. All fluorescence values are normalized on per-cell basis and reported as normalized mean fluorescence ± standard deviation. Two-way ANOVA analysis indicates no statistical difference (p > 0.05) in fluorescence accumulation between E6-derived BMECs and UM-derived BMECs. All substrate and inhibitor conditions were repeated in an additional independent differentiation per medium to confirm reported trends