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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Accelerated differentiation of human induced pluripotent stem cells to blood–brain barrier endothelial cells

Fig. 2

Immunocytochemical analysis of BBB markers before and after purification. a Immunocytochemical detection of PECAM-1 after 4 D E6 medium. Scale bar is 200 μm. b Immunocytochemical detection of GLUT-1, occludin, PECAM-1, and VE-cadherin after 4 D E6 medium and 2 D EC medium supplemented with bFGF and RA. All scale bars are 50 μm. c Immunocytochemical detection of claudin-5, GLUT-1, occludin, PECAM-1, and VE-cadherin following purification and induction of barrier phenotype. All scale bars are 50 μm

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