Fig. 2From: Accelerated differentiation of human induced pluripotent stem cells to blood–brain barrier endothelial cellsImmunocytochemical analysis of BBB markers before and after purification. a Immunocytochemical detection of PECAM-1 after 4 D E6 medium. Scale bar is 200 μm. b Immunocytochemical detection of GLUT-1, occludin, PECAM-1, and VE-cadherin after 4 D E6 medium and 2 D EC medium supplemented with bFGF and RA. All scale bars are 50 μm. c Immunocytochemical detection of claudin-5, GLUT-1, occludin, PECAM-1, and VE-cadherin following purification and induction of barrier phenotype. All scale bars are 50 μmBack to article page