Skip to main content
Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Accelerated differentiation of human induced pluripotent stem cells to blood–brain barrier endothelial cells

Fig. 1

Differentiation of iPSCs to BMECs. iPSCs were seeded at defined densities and differentiated for either 6 days in unconditioned medium (UM) or for 4 days in defined E6 medium. Impure immature BMECs were subsequently treated for 2 days with endothelial cell (EC) medium supplemented with bFGF and retinoic acid (RA). Mature BMECs were purified onto Transwell filters or plates for 24 h in EC medium supplemented with bFGF and RA. 24 h after purification, barrier was induced by treating cells with EC medium lacking bFGF and RA

Back to article page

Fluids and Barriers of the CNS

ISSN: 2045-8118