Fig. 1
From: Permeability across a novel microfluidic blood-tumor barrier model
a Schematic of SynVivo BBB microfluidic chip: (1) inlet port where media with or without tracer is flowed through the outer compartment to change media for HUVECs. (2) Outer compartment, containing HUVECs. (3) 3 μm pores, to allow diffusion of media and tracer between the central and outer compartments. (4) Central compartment, containing astrocytes or cancer cells. (5) Outlet port where perfusate from the outer compartment is collected. (6) Inlet port for central compartment, used to seed and change media for the astrocytes/cancer cells in the central compartment. (7) Output ports where perfusate from the central compartment is collected. b Morphology of astrocytes in the central compartment and HUVECs in the outer compartment without the addition of flow (c) morphology of astrocytes in the central compartment and HUVECs in the outer compartment with the addition of flow. d Representation of where the regions of interest (ROI) measurements are taken for data analysis. White rectangle scale bars 500 μm