Fig. 3

Immunohistochemistry and immunofluorescence staining of GLUT9 in methacarn-fixed murine brain. Paraffin sections of methacarn-fixed murine brain were used for immunostaining. a, b Antigen absorption test. Immunohistochemistry using showing a anti-GLUT9 antibody staining in the third ventricle ependyma and parenchyma and b antigen-preabsorbed antibody. Scale bar 100 µm. c GLUT9 immunoreactivity observed in brain capillaries in the cortex. Scale bar 100 µm. d–f Immunofluorescence staining of d GLUT9 and e P-gp showing co-localization in capillaries. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 µm. P-gp, P-glycoprotein