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Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Immunohistochemical and in situ hybridization study of urate transporters GLUT9/URATv1, ABCG2, and URAT1 in the murine brain

Fig. 3

Immunohistochemistry and immunofluorescence staining of GLUT9 in methacarn-fixed murine brain. Paraffin sections of methacarn-fixed murine brain were used for immunostaining. a, b Antigen absorption test. Immunohistochemistry using showing a anti-GLUT9 antibody staining in the third ventricle ependyma and parenchyma and b antigen-preabsorbed antibody. Scale bar 100 µm. c GLUT9 immunoreactivity observed in brain capillaries in the cortex. Scale bar 100 µm. df Immunofluorescence staining of d GLUT9 and e P-gp showing co-localization in capillaries. Blue indicates DAPI-stained nucleus of the brain capillary endothelial cell. Scale bar 10 µm. P-gp, P-glycoprotein

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